IUPUI biologists, increasing human polypotent cells of cellular cellular cellular cells on the desktop, have created a way to create more mature models that better imitate the environment of human retina. Introducing hPSC-RGCs to astrochets, researchers can create cells more analogous to human RGCs and can be used further to study diseases such as glaucoma. These results are published online News Cell Reports.
Jason Meyer, associate professor of biology at the School of Science at IUPUI, uses the retina derived from HPSC to better understand the development and maturation of cellular cellular cells. These cells transmit visual information to the brain, and when this connection bothers, a person loses sight. But RGC does not exist and works only in the retina; the astro-cellular cells are essential to provide support and instruction to the cellular cellular networks.
"The astrokeum is found in the retina, but very specifically in association with the cellular cellular cells," said Meyer. "They are everywhere around the cellular cellular networks and through the optical nerve connecting to the brain, so we have found that they have an important role in how these RGCs evolve and work."
A biological diplomat and first author of the paper Kirstin B. VanderWall has set up a system to grow RGCs alone or with the astrochets to see how the astrocytes influence the growth and maturity of these cells.
"What we have found is that the astrocytes speed up the difference and provide a retina ganglion, which works more appropriately and acts more like we would expect these cells to function in the human retina," said Meyer.
RGCs are the cells mainly damaged by glaucoma, disease, which is the second main cause of blindness. These results guide development of a more suitable model to study how the cells are affected in diseases such as glaucoma and could lead to a laboratory model of the disease.
"Glaucoma is not developed in immature cells that are still growing, we want to get the cells that we study as close to the stage as they begin to develop problems," said Meyer. "At last, what we have found is that these network cellular cells have acquired some mature characteristics on their own, but we can improve it with astrocytes."
Materials provided by Indiana University-Purdue-University Indianapolis School of Science. Note: Content can be edited for style and length.